Super-Resolution Imaging of Mitochondria and Microtubules
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چکیده
Super-resolution microscopy using the TIRFM (total internal reflection fluorescence microscope) was employed to visualize and elucidate the interactions between mitochondria and microtubules inside the cell. The mitochondria were labeled with the enhanced yellow fluorescent protein (EYFP) through transfection, and the microtubules were labeled with the synthetic dye, Alexa Fluor 647, through immunostaining. The acquired dSTORM images show clear localization of mitochondria and microtubules in healthy cells and may suggest a possible interaction between the two organelles.
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تاریخ انتشار 2013